Preparation of lysocellin beads by fermentation
AssigneeMallinckrodt Veterinary, Inc.
InventorsDonald R. Hahn, James R. McMullen, Vikram P. Mehrotra
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Abstract
A method for producing lysocellin in bead form comprises: (a) cultivating a lysocellin-producing microorganism in an aqueous nutrient-containing fermentation broth under lysocellin-producing conditions; (b) adding a base or inorganic phosphate to the fermentation broth within about the first 24 hours of the beginning of the fermentation in an amount sufficient to ensure that the lysocellin is produced in the form of a cation salt of lysocellin; (c) providing a lipid to the fermentation broth such that the lipid is present in the broth in a sufficient amount to form discrete beads with the lysocellin in the fermentation broth; and (d) separating the beads from the broth.
Claims16
A method for producing lysocellin in bead form which comprises the steps of:
- (a) cultivating a lysocellin-producing microorganism in an aqueous nutrient-containing fermentation broth under lysocellin-producing conditions so as to produce lysocellin;
- (b) adding a precalculated amount of base or inorganic salt to said fermentation broth so as to provide said fermentation broth with a concentration of base or salt of at least about 25 mM within the first 24 hours from the beginning of fermentation such that said lysocellin is in the form of a cation salt;
- (c) providing a lipid to said fermentation broth such that throughout the fermentation said lipid is present in said fermentation broth in an amount which is sufficient to form discrete beads with said cation salt of lysocellin in said fermentation broth;
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- (d) separating said beads from said fermentation broth.
Dependencies are inferred from the claim text.