Substrate material for simultaneously binding genotypic and phenotypic substances
AssigneeEvotec BioSystems AG
InventorsKarsten Henco, Manfred Eigen
Life of this patent
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Abstract
Described is a support material that can simultaneously bind both genotypic and phenotypic substances. The respective areas, which can have surface-modifying matter such as anionic exchangers and/or affinity ligands, simultaneously bind, for example, nucleic acids and proteins or peptides. The support materials described can be used in processes for evolutive optimization of biopolymers, wherein genotype and phenotype can be bound at the same time so as to furnish them for further analysis.
Claims15
A substrate material having at least two simultaneous binding properties for the respective specific binding of nucleic acids and their corresponding expression products, wherein
- a) said substrate material has a first surface area capable of binding only said nucleic acids and, separate from said first surface area, a second surface area capable of binding only said expression products, and one of said first and second surface areas has affinity properties effected by a first affinity ligand or ligands and the other said surface area has ion-exchange or affinity properties effected by a second affinity ligand or ligands, said affinity properties of said first surface area differing from said affinity properties of said second surface area
- or
- b) said substrate material is capable of binding said nucleic acids and said expression products over the entire surface area, said surface area having a first portion with affinity properties effected by a first affinity ligand or ligands and a second portion with ion-exchange or affinity properties effected by a second affinity ligand or ligands, said affinity properties of said first portion differing from said affinity properties of said second portion.
A process comprising the sequential steps of:
- subjecting a nucleic acid to mutagenesis in a reaction chamber to effect a mutagenized nucleic acid;
- subjecting the mutagenized nucleic acid to at least one replication;
- expressing the mutagenized nucleic acid to effect an expression product;
- binding the expression product along with the nucleic acid on a substrate material having at least two simultaneous binding properties for the respective specific binding of nucleic acids and their corresponding expression products, wherein
- a) said substrate material has a first surface area capable of binding only said nucleic acids and, separate from said first surface area, a second surface area capable of binding only said expression products, and one of said first and second surface areas has affinity properties effected by a first affinity ligand or ligands and the other said surface area has ion-exchange or affinity properties effected by a second affinity ligand or ligands, said affinity properties of said first surface area differing from said affinity properties of said second surface area
- or
- b) said substrate material is capable of binding said nucleic acids and said expression products over the entire surface area, said surface area having a first portion with affinity properties effected by a first affinity ligand or ligands and a second portion with ion-exchange or affinity properties effected by a second affinity ligand or ligands, said affinity properties of said first portion differing from said affinity properties of said second portion;
- and
- examining the bound expression product.
Dependencies are inferred from the claim text.