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AssigneeMiller Brewing Company
InventorsMatthew Tripp, Lance Lusk, Thomas Rhodes, Nick Huige, Edward Kot, Etzer Chicoye, Michael C. Barney, Patricia A. Bower, Charles L. Cronan
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Yeast is genetically engineered by transformation with an expression vector containing a natural yeast secretion signal sequence combined appropriately with a chemically synthesized gene encoding antifreeze protein resulting in the expression, proper processing, and secretion of antifreeze protein which is heterologous to yeast in recoverable amounts. Disclosed are DNA sequences comprising structural genes encoding peptides having amino acid sequences with the biochemical or physiochemical properties of antifreeze protein and a method of combining the antifreeze protein gene sequences with appropriate expression vectors.
An expression vector suitable for transformation into S. cerevisiae yeast so that upon such transformation the yeast expresses and secretes an antifreeze protein without a methionine at a N terminus of the protein, the vector comprising a DNA sequence encoding the protein having an amino acid sequence according to SEQ ID NO:1, except that one amino acid is replaced with a different amino acid in SEQ ID NO:1.
Dependencies are inferred from the claim text.