Human growth hormone variants
AssigneeGenentech, Inc.
InventorsBrian C. Cunningham, Henry B. Lowman, James A. Wells, Ross G. Clark, Kenneth Olson, Germaine G. Fuh
Life of this patent
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Abstract
Human growth hormone variants, DNA encoding the variants, vectors, host cells, pegylated forms of the variants, as well as methods of making the variants are disclosed.
Claims22
A human growth hormone variant comprising the following set of amino acid substitutions:
- H18D, H21N, R167N, K168A, D171S, K172R, E174S, I179T.
A nucleic acid encoding a human growth hormone variant comprising the following set of amino acid substitutions:
- H18D, H21N, R167N, K168A, D171S, K172R, E174S, I179T.
A nucleic acid encoding a human growth hormone variant comprising the following set of amino acid substitutions:
- H18D, H21N, R167N, K168A, D171S, K172R, E174S, I179T, and further comprising a substitution at G120.
A vector comprising a nucleic acid encoding a human growth hormone variant comprising the following set of amino acid substitutions:
- H18D, H21N, R167N, K168A, D171S, K172R, E174S, I179T.
A vector comprising a nucleic acid encoding a human growth hormone variant comprising the following set of amino acid substitutions:
- H18D, H21N, R167N, K168A, D171S, K172R, E174S, I179T, and further comprising a substitution at G120.
A host cell comprising a vector comprising a nucleic acid encoding a human growth hormone variant comprising the following set of amino acid substitutions:
- H18D, H21N, R167N, K168A, D171S, K172R, E174S, I179T.
A host cell comprising a vector comprising a nucleic acid encoding a human growth hormone variant comprising the following set of amino acid substitutions:
- H18D, H21N, R167N, K168A, D171S, K172R, E174S, I179T, and further comprising a substitution at G120.
A process for preparing a human growth hormone variant comprising culturing a host cell comprising a vector comprising a nucleic acid encoding a human growth hormone variant comprising the following set of amino acid substitutions:
- H18D, H21N, R167N, K168A, D171S, K172R, E174S, I179T and recovering the human growth hormone variant from the culture.
A process for preparing a human growth hormone variant comprising culturing a host cell comprising a vector comprising a nucleic acid encoding a human growth hormone variant comprising the following set of amino acid substitutions:
- H18D, H21N, R167N, K168A, D171S, K172R, E174S, I179T, and further comprising a substitution at G120 and recovering the human growth hormone variant from the culture.
A nucleic acid encoding a human growth hormone variant comprising the following set of amino acid substitutions:
- H18D, H21N, G120K, R167N, K168A, D171S, K172R, E174S, I179T.
A vector comprising a nucleic acid encoding a human growth hormone variant comprising the following set of amino acid substitutions:
- H18D, H21N, G120K, R167N, K168A, D171S, K172R, E174S, I179T.
A host cell comprising a vector comprising a nucleic acid encoding a human growth hormone variant comprising the following set of amino acid substitutions:
- H18D, H21N, G120K, R167N, K168A, D171S, K172R, E174S, I179T.
Dependencies are inferred from the claim text.