DNA encoding leukocyte derived growth factor-2 (LDGF-2)
AssigneeUniversity of South Florida
InventorsGary R. Grotendorst, Naoko Iida
Life of this patent
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Abstract
A protein, Leukocyte Derived Growth Factor 2 (hereinafter LDGF2) having PDGF-like activity is described. LDGF2 reacts with PDGF receptors and possesses mitogenic and/or chemotactic activity for various cell types, particularly connective tissue cells. LDGF2 may be used as the active ingredient in therapeutic compositions, e.g. wound healing compositions, or even further may be used as an additive to cell culture media for the purpose of stimulating cell growth. The protein has a molecular weight of about 7000 daltons determined by SDS gel electrophoresis and is about 61 amino acids in length.
Claims24
An isolated nucleic acid having a nucleotide sequence coding for LDGF2.
An isolated nucleic acid having a nucleotide sequence coding for LDGF2 said LDGF2 having immunoreactivity.
A host cell transformed to express LDGF2, said LDGF2 having immunoreactivity.
A method of producing LDGF2 having immunoreactivity comprising:
- culturing a host cell transformed with a nucleic acid vector directing expression of a nucleotide sequence coding for LDGF2 under conditions such that expression of LDGF2 occurs;
- and
- isolating the expressed LDGF2 having immunoreactivity from the culture.
Dependencies are inferred from the claim text.