Use of linalool synthase in genetic engineering of scent production
AssigneeThe Regents of the University of Michigan
InventorEran Pichersky
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Abstract
A purified S-linalool synthase polypeptide from Clarkia breweri is disclosed as is the recombinant polypeptide and nucleic acid sequences encoding the polypeptide. Also disclosed are antibodies immunoreactive with the purified peptide and with recombinant versions of the polypeptide. Methods of using the nucleic acid sequences, as well as methods of enhancing the smell and the flavor of plants expressing the nucleic acid sequences are also disclosed.
Claims32
An isolated nucleic acid segment comprising a nucleic acid sequence encoding a linalool synthase protein or polypeptide.
An isolated nucleic acid segment which comprises a sequence of at least 15 contiguous nucleotides which corresponds to at least a 15 nucleotide contiguous sequence of SEQ ID NO:1 or its complement.
A method of using a nucleic acid segment encoding a linalool synthase protein or polypeptide, comprising the steps of:
- preparing a recombinant vector in which said nucleic acid segment is positioned under the control of a promoter;
- introducing said recombinant vector into a host cell;
- and
- culturing said host cell under conditions effective to allow expression of the encoded linalool synthase protein or polypeptide.
Dependencies are inferred from the claim text.