Enhancer for eukaryotic expression systems
AssigneeHoechst Aktiengesellschaft
InventorsBernhard Fleckenstein, Walter Schaffner, Frank Weber, Karoline Dorsch-Hasler, Gerhard Jahn, Michael Boshart
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Abstract
An enhancer has been located in the upstream region of the major immediate early gene of human cytomegalovirus and has been isolated, which enhancer is more active than that from SV40 and has a wide host cell spectrum. Hence, it is suitable for eukaryotic expression systems wherein it can be incorporated upstream or downstream of the structural gene or of the regulation region.
Claims4
The method to increase expression of a gene in a mammalian cell comprising inserting into a mammalian cell an isolated DNA enhancer consisting of DNA from the upstream region of the major immediate early (IE) gene of human cytomegalovirus (HCMV) and a heterologous gene that is to be expressed, wherein the DNA from the upstream region of the IE gene of HCMV is the only HCMV material to which the mammalian cell is exposed.
Dependencies are inferred from the claim text.