Method of identifying agents that modulate UCP2 promoter activity
AssigneeTularik Inc.
InventorsM. Catherine Amaral, Jin-Long Chen
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Abstract
The invention relates to regulators of UCP2 gene transcription, including novel UCP2 transcriptional promoters. UCP2 gene promoters are used in diagnosis and pharmaceutical development. In particular, transfected cells comprising UCP2 gene promoters operably linked to a reporter are used in high-throughput pharmaceutical screens.
Claims23
A method for identifying an agent which modulates the association of a UCP2 promoter and a transcription factor, the method comprising steps:
- combining a regulator of gene expression comprising a nucleotide sequence which consists of nucleotides 1 to 460 of SEQ ID NO:1 or a fragment of the nucleotide sequence which is at least 50 nucleotides in length and has cis transcriptional regulatory activity, said transcription factor and a candidate agent, under conditions wherein, but for the presence of the candidate agent, the regulator and the transcription factor form a first association;
- and
- detecting the presence of a second association of the regulator and the transcription factor,
- wherein a difference between the first and the second association indicates that the candidate agent is an agent that modulates the association of the UCP2 promoter and the transcription factor.
A method for identifying an agent which modulates the association of a UCP2 promoter and a transcription factor and thereby regulates the activity of a UCP2 promoter, the method comprising steps:
- combining a cell comprising a regulator of gene expression comprising a nucleotide sequence which consists of nucleotides 1 to 460 of SEQ ID NO:1 or a fragment of the nucleotide sequence which is at least 50 nucleotides in length, comprises at least one sequence selected from the group consisting of SEQ ID NO:1, nucleotides 411-460, 461-510, 401-563, 319-326, 98-104, 49-56, 49-104 and 547-554 and has cis transcriptional regulatory activity operably linked to a non-UCP2 gene, with a candidate agent, under conditions wherein, but for the presence of the candidate agent, the gene exhibits a first expression;
- and
- detecting the presence of a second expression of the gene,
- wherein a difference between the first and the second expression indicates that the candidate agent is an agent that modulates the association of the UCP2 promoter and the transcription factor and thereby regulates the activity of the UCP2 promoter.
Dependencies are inferred from the claim text.