Mutant proteins of human DNA topoisomerase I
AssigneeNippon Hoechst Marion Roussel Limited
InventorsMasako Wagatsuma, Noriko Kurita
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Abstract
Mutant proteins of human DNA topoisomerase I having an amino acid sequence in which tyrosine at the 592nd position of human DNA topoisomerase I is lacking or replaced with phenylalanine and which contains at least 30 amino acids in succession of the amino acid sequence subsequent to the 542nd amino acid of human DNA topoisomerase I. Above described mutant proteins react with anti-Scl-70, antibody in the sera of autoimmune disease patients of diffuse scleroderma and can be produced by genetic engineering using E. coli, and therefore they are useful as a diagnostic agent of scleroderma.
Claims19
A mutant human DNA topoisomerase I protein comprising the amino acid sequence from the 96th through the 590th of the amino acid sequence described in SEQ ID No.:1.
A mutant of human DNA topoisomerase I protein comprising the amino acid sequence from the 591st through the 765th of the amino acid sequence described in SEQ ID No.:1.
A mutant human DNA topoisomerase I protein comprising the amino acid sequence from the 96th through the 765th of the amino acid sequence described in SEQ ID No.:1.
A mutant human DNA topoisomerase I protein comprising the amino acid sequence from the 542nd through the 765th of the amino acid sequence described in SEQ ID No.:1.
A mutant human DNA topoisomerase I protein comprising the amino acid sequence from the 1st through the 765th of the amino acid sequence described in SEQ ID No.:1.
A mutant human DNA topoisomerase I protein comprising the amino acid sequence from the 1st through the 765th of the amino acid sequence described in SEQ ID No.:1, in which the amino acids at the 592nd position and 723rd position are both lacking.
A mutant human DNA topoisomerase I protein comprising an amino acid sequence from the 542nd amino acid through the 592nd amino acid of the amino acid sequence described in SEQ ID NO:1.
A mutant human topoisomerase I protein comprising an amino acid sequence from the 542nd amino acid through the 592nd amino acid of the amino acid sequence described in SEQ ID NO:
- 1, wherein the amino acid at the 592nd position is lacking.
A mutant human DNA topoisomerase I protein comprising an amino acid sequence from the 1st through the 765th amino acid of the amino acid sequence described in SEQ ID NO:1, in which one amino acid at the 592nd position is lacking and the amino acid at the 723rd position is replaced with tyrosine.
Dependencies are inferred from the claim text.