TNF receptor death domain ligand proteins and method to identify inhibitors of ligand binding
AssigneeGenetics Institute, Inc.
InventorsLih-Ling Lin, Jennifer Chen, Andrea R. Schievella
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Abstract
Novel TNF receptor death domain ("TNF-R1-DD") ligand proteins are disclosed. Polynucleotides encoding the TNF-R1-DD ligand protein are also disclosed, along with vectors, host cells, and methods of making the TNF-R1-DD ligand protein. Pharmaceutical compositions containing the TNF-R1-DD ligand protein, methods of treating inflammatory conditions, and methods of inhibiting TNF-R death domain binding are also disclosed. Methods of identifying inhibitors of TNF-R death domain binding and inhibitors identified by such methods are also disclosed.
Claims6
A method of identifying an inhibitor of TNF-R1 death domain binding which comprises:
- (a) combining a TNF-R1 death domain protein with a composition comprising a TNF-R1-DD intracellular ligand protein, said combination forming a first binding mixture;
- (b) measuring the amount of binding between the TNF-R1 death domain protein and the TNF-R1-DD ligand protein in the first binding mixture;
- (c) combining a compound with the TNF-R1 death domain protein and a TNF-R1-DD intracellular ligand protein to form a second binding mixture;
- (d) measuring the amount of binding in the second binding mixture;
- and
- (e) comparing the amount of binding in the first binding mixture with the amount of binding in the second binding mixture;
- wherein the compound is capable of inhibiting TNF-R1 death domain binding when a decrease in the amount of binding of the second binding mixture occurs.
Dependencies are inferred from the claim text.