Phagemid for antibody screening
AssigneeDeutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts
InventorsFrank Breitling, Melvyn Little, Stefan Dubel, Michael Braunagel, Iris Klewinghaus
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Abstract
A phagemid has been constructed that expresses an antibody fused to coliphage pIII protein. The phagemid is suitable for selecting specific antibodies from large gene libraries with small quantities of antigen. The antibody-pIII gene can be strongly repressed, so that it allows antibody libraries to be amplified without the danger of deletion mutants predominating. After induction, large quantities of the fusion protein may be expressed.
Claims7
A phagemid comprising a DNA encoding a single-chain antibody-coliphage pIII fusion protein, wherein the fusion protein contains a contiguous full length coliphage pIII protein.
A method of presenting a peptide or protein at the surface of a phagemid viral particle, comprising producing said phagemid viral particle, wherein said phagemid viral particle comprises a DNA sequence encoding said peptide or protein fused to a DNA sequence encoding a contiguous full length coliphage pIII protein.
Dependencies are inferred from the claim text.