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US 5,849,499 AGranted
Monoclonal antibody to acetylated lysine residues in recombinant bovine somatotropin
AssigneePharmacia & Upjohn Company
InventorsDiane Marie Hamlin, Gustavus Adolphus Walker
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Abstract
Provided are hybridomas for producing monoclonal antibodies against acetylated lysine residues of rbSt. The antibodies are specific to the presence of at least a single acetylated lysine in a protein and can differentiate between .alpha.-acetyl and .epsilon.-acetyl lysine as free amino acids.
Claims5
A method of determining the percentage of acetylation in recombinant bovine somatotropin comprising the steps of:
- a) contacting monoclonal antibodies against a recombinant bovine somatotropin having acetylated lysine residues with a recombinant bovine somatotropin sample;
- b) maintaining the monoclonal antibodies in contact with the recombinant bovine somatotropin for a time and under conditions sufficient to allow formation of immunological complexes between the monoclonal antibodies and the recombinant bovine somatotropin;
- and
- c) detecting the quantity of the immunological complexes.
1 more element
A hybridoma which is ATCC HB-10181.
A monoclonal antibody produced by ATCC HB-10181.
Dependencies are inferred from the claim text.