DNA-sequence-based diagnosis of mastitis from a milk sample
AssigneeOulutech Ltd.
InventorsJouko Tapani Alatossava, Paivi Tuulikki Forsman, Anu Kyllikki Tilsala-Timisjarvi
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Abstract
A rapid method for diagnosing mastitis is described. The method is based on DNA sequence identification comprising the steps of determining the presence of the following DNA sequences in a milk sample: a DNA sequence specific for somatic cells for indicating inflammation; a DNA sequence specific for a mastitis pathogen for indicating infection; and a DNA sequence specific for an antibiotic-resistance-encoding gene of a pathogen for assisting a proper drug therapy. Test kits and genus- or species-specific oligonucleotides and their use in said method are also described.
Claims13
A method for diagnosing mastitis comprising the steps of determining the presence of each of the following DNA sequences in a milk sample:
- a somatic cell-specific DNA sequence whose presence indicates inflammation;
- a mastitis pathogen-specific DNA sequence whose presence indicates infection by said pathogen;
- a DNA sequence of at least one antibiotic resistance-encoding gene of a mastitis pathogen whose presence may be used to determine a proper drug therapy to treat mastitis;
- wherein the presence of each of said DNA sequences is determined by contacting nucleic acids in said sample with oligonucleotides specifically hybridizing with said DNA sequences and detecting any hybrids formed, thereby providing a diagnosis of mastitis.
A test kit for use in a method for diagnosing mastitis from a milk sample comprising at least the following oligonucleotides:
- an oligonucleotide which specifically hybridizes with a somatic cell-specific DNA sequence;
- an oligonucleotide which specifically hybridizes with a mastitis pathogen-specific DNA sequence;
- an oligonucleotide which specifically hybridizes with a DNA sequence of an antibiotic resistance-encoding gene of a mastitis pathogen.
An oligonucleotide specifically hybridizing with a sequence of any of SEQ ID NO 2 to 11 or with a sequence of the complementary strands thereof.
Dependencies are inferred from the claim text.