High throughput screening method for sequences or genetic alterations in nucleic acids
AssigneeIG Laboratories, Inc.
InventorAnthony P. Shuber
Life of this patent
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Abstract
A high-throughput method for screening nucleic acid samples to identify target sequences or one or more genetic alterations in target sequences present in the nucleic acid samples. Methods of identifying target nucleic acid sequences in patient samples, and of identifying randomly permuted alterations in nucleic acid sequences of interest are also included.
Claims25
A method for screening nucleic acid samples to identify one or more genetic alterations of interest in one or more target sequences present in said samples, comprising the steps of:
- (i) immobilizing a plurality of said samples on a support;
- (ii) simultaneously hybridizing said samples with a pool of polymer probes
- wherein said pool comprises individual purine and pyrimidine containing polymers, each polymer complementary to and specific for one of said one or more genetic alterations of interest suspected to be present in said target sequences;
- (iii) removing the purine and pyrimidine containing polymers that do not hybridize to said samples;
- (iv) detecting the presence of polymer:sample hybrids;
- (v) individually separating said purine and pyrimidine containing polymers hybridized to said samples;
- and
- (vi) identifying said separated purine and pyrimidine containing polymers
- wherein the identification of said separated purine and pyrimidine containing polymers identifies the nucleic acid sequence of said genetic alteration(s).
A method for identifying one or more target sequences of interest present in a nucleic acid sample, comprising the steps of:
- (i) immobilizing a plurality of nucleic acid samples on a support;
- (ii) simultaneously hybridizing said samples with a pool of polymer probes
- wherein said pool comprises individual purine and pyrimidine containing polymers, each polymer complementary to and specific for one of said one or more target sequences of interest suspected to be present in said samples;
- (iii) removing the purine and pyrimidine containing polymers that do not hybridize to said samples;
- (iv) detecting polymer:sample hybrids;
- (v) individually separating said polymers hybridized to each of said samples;
- and
- (vi) determining the sequence of said individually separated purine and pyrimidine containing polymers
- wherein the sequence identifies the nucleic acid sequence of said one or more targets present in said sample.
A method for identifying one or more randomly permuted alteration of interest in a target sequence present in a nucleic acid sample, comprising the steps of:
- (i) immobilizing a plurality of nucleic acid samples on a support;
- (ii) simultaneously hybridizing said samples with a pool of polymer probes
- wherein said pool comprises individual purine and pyrimidine containing polymers, each polymer complementary to and specific for one of said one or more randomly permuted alterations of interest suspected to be present in said target sequence;
- (iii) removing the purine and pyrimidine containing polymers that do not hybridize to said samples
- (iv) detecting the presence of polymer:sample hybrids;
- (v) individually separating each of said purine and pyrimidine containing polymers hybridized to each of said samples;
- and
- (vi) identifying said separated purine and pyrimidine containing polymers
- wherein the identification of said separated purine and pyrimidine containing polymers identifies said randomly permuted alteration (s) in said target sequence.
Dependencies are inferred from the claim text.