Recombinant varicella-zoster virus and process for constructing same
AssigneeThe Research Foundation for Microbial Diseases of Osaka University
InventorsKimiyasu Shiraki, Michiaki Takahashi
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Abstract
The present invention provides a recombinant varicella-zoster virus prepared by inserting, into the viral genome, nucleic acids from the hepatitis B virus genome, a genomic DNA of the recombinant varicella-zoster virus, a live vaccine containing the recombinant varicella-zoster virus as an effective ingredient, an antigen derived from the recombinant varicella-zoster virus, and diagnostic agent containing the antigen. The recombinant varicella-zoster virus of the present invention can be utilized as a multivalent vaccine having an excellent immunity effect both on chicken pox and hepatitis B, and expression products and genomic DNA thereof may be used as a multivalent diagnostic agent.
Claims6
An antigenic hepatitis B virus protein, which is obtained from a cell infected with a recombinant varicella-zoster virus or is obtained from a culture supernatant of said infected cell, wherein the recombinant varicella-zoster virus comprises the genomic DNA of the Oka varicella vaccine strain and the HpaII fragment of the hepatitis B surface antigen gene, wherein said fragment is inserted in the HincII site of the thymidine kinase gene of said genomic DNA, so that the 5'-end of said fragment is linked with the starting codon of said thymidine kinase gene in a correct reading frame, and the hepatitis B surface antigen is expressed under sole control of said thymidine kinase gene promoter.
Dependencies are inferred from the claim text.