Recombinant vaccinia virus expressing human retrovirus gene
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsBernard Moss, Sekhar Chakrabarti
Life of this patent
Estimating the term…
Abstract
A recombinant vaccinia virus capable of expressing HTLV-III envelope protein (env) has been constructed. The expressed env protein is recognized by sera obtained from AIDS patients. The synthesized env protein also produces antibodies when administered to a suitable host, such antibodies having specific binding affinity with the env protein.
Claims20
A recombinant protein expressed by an HIV envelope gene incorporated in a recombinant vaccinia virus, the protein being expressed in a host cell infected with the recombinant vaccinia virus, the protein being a native HIV envelope protein, being free of non-envelope HIV proteins and being free of HIV virus.
An immunogenic composition comprising one or more recombinant proteins expressed by an HIV envelope gene incorporated in a recombinant vaccinia virus, the one or more proteins being a native HIV envelope protein and being expressed in a host cell infected with the recombinant vaccinia virus, the composition being capable of causing an immunologic reaction with sera from AIDS patients, being free of non-envelope HIV proteins, and being free of HIV virus.
A composition comprising one or more recombinant proteins expressed by an HIV envelope gene incorporated in a recombinant orthopox virus, the protein or proteins being a native HIV envelope protein, being expressed in a host cell infected with the recombinant orthopox virus, and being free of non-envelope HIV proteins and being free of HIV virus.
A method for production of an HIV envelope protein comprising incorporating an HIV envelope gene into a recombinant vaccinia virus, transfecting host cells with the recombinant vaccinia virus carrying the HIV envelope gene and obtaining the HIV envelope protein.
Dependencies are inferred from the claim text.