Production of nitro-benzyl-dota via direct peptide cyclization
AssigneeNeoRx Corporation
InventorsEric K. Yau, Louis J. Theodore, Linda M. Gustavson
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Abstract
Methods, compounds, compositions and kits that relate to pretargeted delivery of diagnostic and therapeutic agents are disclosed. In particular, methods for radiometal labeling of biotin, as well as related compounds, are described. Articles of manufacture useful in pretargeting methods are also discussed.
Claims11
A method of producing nitro-benzyl-DOTA via direct peptide cyclization comprising;
- treating an acyclic tetrapeptide which has an N-terminal amino group and a C-terminal carboxyl group with an activating agent which provides for said carboxyl group of said acyclic tetrapeptide to become susceptible to closure by said amino group;
- and allowing said acyclic tetrapeptide to cyclize to form a cyclic tetraamide.
A method of producing nitro-benzyl-DOTA via direct peptide cyclization comprising:
- treating an acyclic tetrapeptide which has an N-terminal amino group and a C-terminal carboxyl group with an activating agent which provides for said carboxyl group of said acyclic tetrapeptide to become susceptible to closure by said amino group;
- and allowing said acyclic tetrapeptide to cyclize to form a cyclic tetraamide;
- wherein said activating agent is benzotriazolyloxytris(di-methylamino)phosphonium hexafluorophosphate.
A method of producing nitro-benzyl-DOTA via direct peptide cyclization comprising:
- treating an acyclic tetrapeptide which has an N-terminal glycyl amino group and a C-terminal p-nitrophenylalanyl carboxyl group with an activating agent which provides for said carboxyl group of said acyclic tetrapeptide to become susceptible to closure by said amino group;
- and
- allowing said acyclic tetrapeptide to cyclize to form a cyclic tetraamide.
A method of producing nitro-benzyl-DOTA via direct peptide cyclization comprising:
- treating an acyclic tetrapeptide which has an N-terminal glycyl amino group and a C-terminal p-nitrophenylalanyl carboxyl group with an activating agent which provides for said carboxyl group of said acyclic tetrapeptide to become susceptible to closure by said amino groups;
- and
- allowing said acyclic tetrapeptide to cyclize to form a cyclic tetraamide;
- wherein said activating agent is benzotriazolyloxytris(di-methylamino)phosphonium hexafluorophosphate.
Dependencies are inferred from the claim text.