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US 5,847,078 AGranted
Polypeptide capable of differentiating and maturing malignant cells into normal cells or capable of accelerating the formulation of erythroblasts
OwnerYuzuru Eto, Tomoko Tsuji +4inventors
InventorsYuzuru Eto, Tomoko Tsuji, Satoshi Takano, Misako Takezawa, Yasunori Yokogawa, Hiroshiro Shibai
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Abstract
A physiologically active polypeptide, BUF-3, having the ability to differentiate and maturate human leukemia cells into normal cells and of accelerating the formation of erythroblasts is disclosed. The polypeptide has a molecular weight of 16.+-.1 Kd as determined by SDS-electrophoresis in the presence of 1% mercaptoethanol, or 25.+-.1 Kd, as determined by SDS-electrophoresis in the absence of mercaptoethanol.
Claims1
A polypeptide having the following characteristics,
- (1) Molecular weight
- 16.+-.1 Kd (SDS-electrophoresis in the presence of 1.0% mercaptoethanol)
- 25.+-.1 Kd (SDS-electrophoresis in the absence of mercaptuethanol
- (2) Isoelectric point
- pI 6.3.+-.0.2 (chromatofocusing)
- pI 7.3 (electrofocusing)
- (3) pH stability
- Stable within pH range between 2.0 and 10.0
- (4) Thermal stability
- (4) Thermal stability
- Stable against heating at 65.degree. C. for 60 minutes
- (5) Stability against organic solvents
- Stable against lower alcohols and acetonitrile
- (6) Resistance to protease
- Becomes completely deactivated by treatment with Pronase
- (7) Amino acid sequence at amino terminal ##STR3## (wherein X is a naturally occurring amino acid) (8) Amino acid sequence of peptide fragment formed by cyanogen bromide
- Leu-Tyr-Tyr-Asp-Asp-Gly-Gln-Asn-Ile-Ile-Lys-Lys-Asp-Ile-Gln-Asn
- and wherein said polypeptide is expressed when human malignant leukemia cells selected from the group consisting of K-562, THP-1 and J-111 are cultured in a medium to which 12-0-tetradecanoylphorbol-13-acetate is added.
15 more elements
Dependencies are inferred from the claim text.