Method for eliciting an immune response using a gene expression system that co-delivers an RNA polymerase with DNA
AssigneeOhio University Edison Animal Biotechnology Institute
InventorsThomas E. Wagner, Xiaozhuo Chen, Yunsheng Li
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Abstract
The present invention relates to a gene expression system in eukaryotic cells. In particular, it relates to a self-initiating and self-sustaining gene expression system utilizing an RNA polymerase prebound to a DNA construct before the introduction of the complex into cells. This system is capable of functioning in the cell cytoplasm without integration into the genome of host cells, and does not require host cell factors for initiation and maintenance of gene expression. Therefore, the invention has a wide range of applications in in vitro and in vivo gene expression, including gene therapy in resting cells.
Claims13
A method for eliciting an immune response to an antigen in a mammal, comprising:
- (a) co-delivering into the mammal:
- (i) a recombinant DNA molecule which comprises a coding sequence for an RNA polymerase having transcriptional activity that does not require mammalian host cell factors or a functional derivative thereof, operably linked to its cognate promoter;
- (ii) a second recombinant DNA molecule which comprises a coding sequence for a gene that encodes the antigen or a functional derivative thereof, operably linked to a second copy of the cognate promoter of (i);
- and
- (iii) an exogenous RNA polymerase which transcribes said coding sequences from said cognate promoters;
- and
- (b) eliciting an immune response to the antigen expressed by the gene in the mammal.
A method for eliciting an immune response to an antigen in a mammal, comprising:
- (a) co-delivering into the mammal:
- (i) a recombinant DNA molecule which comprises a coding sequence for an RNA polymerase having transcriptional activity that does not require mammalian host cell factors or a functional derivative thereof, operably linked to its cognate promoter, and a coding sequence for a gene that encodes the antigen or a functional derivative thereof, operably linked to a second copy of the cognate promoter;
- and
- (ii) an exogenous RNA polymerase which transcribes said coding sequences from said cognate promoters;
- and
- (b) eliciting an immune response to the antigen expressed by the gene in the mammal.
A method for eliciting an immune response to an antigen in a mammal, comprising:
- (a) co-delivering into the mammal:
- (i) a recombinant DNA molecule which comprises a coding sequence for a gene of interest or a functional derivative thereof, operably linked to a cognate promoter for an RNA polymerase having transcriptional activity that does not require mammalian host cells factors;
- and
- (ii) an exogenous RNA polymerase which transcribes said coding sequence from said cognate promoter;
- and
- (b) eliciting an immune response to the antigen expressed by the gene in the mammal.
Dependencies are inferred from the claim text.