Methods for hard-tagging an encoded synthetic library

A library of target compounds bound to a solid support wherein each support comprises multiple copies of a single target compound covalently attached thereto wherein each target compound in the library is prepared in situ on the solid support by sequentially conducting at least two reactions wherein at least one of the reactions conducted to prepare a first target compound is different from the reactions conducted to prepare the remaining target compounds and further wherein each of the reactions conducted to prepare each target compound and/or the step in the chemical synthesis where said reaction is conducted is encoded by a tag coupled to the support having said compound bound thereto, immediately before or after coupling of each monomer, wherein said tag is an amine selected from a plurality of amines of formula I:

• ##STR19## or a combination of at least two amines selected from a plurality of amines of formula I:
• ##STR20## wherein R and R' are independently hydrocarbyl groups from 1 to 30 carbon atoms which define a unique amine tag used to identify the reaction conducted in target compound synthesis and/or the point in time where said reaction is conducted;
• R.sup.4 and R.sup.5 are hydrogen;
• and Pg is selected from hydrogen;
• an amine tag of formula I;
• and a compatible protecting group provided that the compatible protecting group is orthogonal to any and all protecting groups employed in compound synthesis,
• provided further that each tag combination employed to encode a single reaction is different from and distinguishable over all other tag combinations used to encode other variations used in that reaction and still further provided that the amine compounds used to encode a first reaction are different from the amine compounds used to encode the other encrypted reactions so as provide a binary or higher coding system.

A method for tagging a solid support having multiple copies of a single target compound covalently attached thereto in order to determine the structure of the target compound attached to the support wherein the compound is prepared in situ on the support by sequentially conducting n reactions on said support wherein n is an integer greater than 1 which method comprises:

• a) conducting a first reaction on the solid support wherein the first reaction and/or the step in the chemical synthesis where said reaction is conducted is encoded by a tag coupled to the support, immediately before or after coupling of each monomer, wherein said tag is an amine selected from a plurality of amines of formula I:
• ##STR21## or a combination of at least two amines selected from a plurality of amines of formula I:
• ##STR22## wherein R and R' are independently hydrocarbyl groups from 1 to 30 carbon atoms which define a unique amine tag used to identify the reaction conducted in target compound synthesis and/or the point in time where said reaction is conducted;
• R.sup.4 and R.sup.5 are hydrogen;
• and Pg is selected from hydrogen;
• an amine tag of formula I;
• and a compatible protecting group provided that the compatible protecting group is orthogonal to any and all protecting groups employed in compound synthesis, provided that one of the Pg groups is hydrogen
• provided that each tag combination employed to encode a single reaction is different from and distinguishable over all other tag combinations used to encode other variations used in that reaction and still further provided that the amine compounds used to encode a first reaction are different from the amine compounds used to encode the other encrypted reactions so as provide a binary or higher coding system;
• and
• b) repeating procedure a) above until all n steps for target compound synthesis on the support are completed.