Polypeptides having kinase activity, their preparation and use
AssigneeLudwig Institute for Cancer Research
InventorsIan D. Hiles, Michael J. Fry, Ritu Dhand, Michael D. Waterfield, Peter J. Parker, Masayuki Otsu, George Panayoutou, Stefano Volinia, Ivan Gout
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Abstract
This invention relates to new polypeptides which exhibit kinase activity or, more specifically, which show phosphoinositide (PI) 3-kinase activity. Such polypeptides are involved in pathways responsible for cellular growth and differentiation. An isolated polypeptide which possesses PI3-kinase activity when produced by recombinant production in insect cells is disclosed.
Claims8
An isolated nucleic acid molecule which encodes the catalytic (110 kD) subunit of PI-3 kinase, selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 32,
- (b) the nucleotide sequence set forth in SEQ ID NO:
- 35, and
- (c) a nucleotide sequence which hybridizes to the complement of at least one of (a) and (b).
Isolated nucleic acid molecule consisting of any one of
- (a) nucleotides 487-525 of SEQ ID NO:
- 32,
- (b) nucleotides 876-1011 of SEQ ID NO:
- 32,
- (c) nucleotides 1321-1392 of SEQ ID NO:
- 32,
- (d) nucleotides 1864-1944 of SEQ ID NO:
- 32,
- (e) nucleotides 1969-2016 of SEQ ID NO:
- 32,
- (f) nucleotides 2035-2097 of SEQ ID NO:
- 32,
- (g) nucleotides 2134-2160 of SEQ ID NO:
- 32,
- (h) nucleotides 2602-2646 of SEQ ID NO:
- 32,
- (i) nucleotides 2653-2724 of SEQ ID NO:
- 32,
- (j) nucleotides 2773-2823 of SEQ ID NO:
- 32,
- (k) nucleotides 2845-2898 of SEQ ID NO:
- 32,
- (l) nucleotides 2959-3030 of SEQ ID NO:
- 32,
- (m) nucleotides 3091-3189 of SEQ ID NO:
- 32, and
- (n) nucleotides 3163-3189 of SEQ ID NO:
- 32.
Dependencies are inferred from the claim text.