Pectate lyase signal sequence
AssigneeXoma Corporation
InventorsRandy R. Robinson, Alvin Y. Liu, Arnold H. Horowitz, Marc Better, Randolph Wall, Shau-Ping Lei, Gary L. Wilcox
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Abstract
The invention is directed to the pectate lyase B secretion signal and its use to express operably linked sequences in bacterial hosts.
Claims21
A purified DNA molecule comprising DNA encoding a pectate lyase pelB secretion signal that is not operably linked to DNA encoding the mature pectate lyase naturally associated with said secretion signal.
A recombinant vector comprising a DNA sequence encoding a pectate lyase pelB secretion signal not operably linked to DNA encoding the mature pectate lyase naturally associated with said secretion signal.
The recombinant vector of any one of claim, 4 or 5-7, wherein said DNA encoding said secretion signal is operably Linked to a promoter.
A method for producing a protein in a bacterial host, said method comprising,
- (a) providing, to said host cell, a DNA encoding a pectate lyase pelB secretion signal operably linked to DNA other than DNA encoding the mature pectate lyase naturally associated with said secretion signal;
- (b) culturing said host cell;
- and
- (c) expressing said protein.
Dependencies are inferred from the claim text.