Gene participating in the mechanism of secretion of esterase
AssigneeTanabe Seiyaku Co., Ltd.
InventorsTakeji Shibatani, Hiroyuki Akatsuka, Eri Kawai
Life of this patent
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Abstract
A gene encoding a polypeptide which participates in the mechanism of secretion of esterase originated from a microorganism of the genus Serratia, a recombinant plasmid comprising a plasmid prepared by inserting said gene into a vector plasmid, a microorganism transformed with said recombinant plasmid, and a method for the production of an esterase which comprises cultivating the transformant microorganism as set forth above in a medium and collecting the produced esterase outside and inside the cells. Said transformed microorganism have remarkably excellent capability of extracellular secretion of esterase.
Claims9
An isolated esterase secretory gene encoding a polypeptide which is required for secretion of esterase originated from a microorganism of the genus Serratia.
A transformant microorganism which is obtained by transforming a host microorganism with a recombinant plasmid comprising a plasmid prepared by inserting a gene encoding an esterase originated from a microorganism of the genus Serratia and a gene encoding a polypeptide which secretion of esterase originated from a microorganism of the genus Serratia into a vector plasmid.
A transformant microorganism which is obtained by transforming a host microorganism with a recombinant plasmid comprising a plasmid prepared by inserting a gene encoding an esterase originated from a microorganism of the genus Serratia into a vector plasmid and a recombinant plasmid comprising a plasmid prepared by inserting a gene encoding a polypeptide which is required for secretion of esterase originated from a microorganism of the genus Serratia into a vector plasmid.
Dependencies are inferred from the claim text.