A biologically pure culture of a microorganism, Alcaligenes latus D2271 (FERM BP-4578), which has been deposited in National Institute of Bioscience and Human-Technology Agency of Industrial Science and Technology, 1-3, Higashi, 1 chome, Tsukuba-shi, Ibaraki-ken 305, Japan, and is capable of forming a maltohexaose a maltoheptaose-forming amylase which has an activity of mainly forming maltohexaose and maltoheptaose from starch, but does not substantially have an activity of hydrolyzing maltohexaose and a lower molecular oligosaccharide than maltohexaose.