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US 5,846,807 AGranted
Media compositions for three-dimensional mammalian tissue growth under microgravity culture conditions
AssigneeThe United States of America as represented by the Administrator of the National Aeronautics and Space Administration
InventorThomas J. Goodwin
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Abstract
Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural and blood tissue. The cells are grown in vitro under microgravity culture conditions and form three dimensional cells aggregates with normal cell function. The microgravity culture conditions may be microgravity or simulated microgravity created in a horizontal rotating wall culture vessel.
Claims10
A culture medium for non-neoplastic mammalian organ tissue culture comprising:
- (a) minimal essential medium-alpha (MEM-ALPHA.TM.);
- (b) Leibovitz L-15.TM. medium;
- (c) tri-sugar supplement;
- and
- (d) serum.
2 more elements
Dependencies are inferred from the claim text.