L-ribose isomerase, its preparation and uses
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsKen Izumori, Keiji Tsusaki
Life of this patent
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Abstract
An L-ribose isomerase which isomerizes aldoses such as L-ribose, D-lyxose, D-talose, D-mannose, L-allose and L-gulose into their corresponding ketoses such as L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose. The enzymatic reaction is a reversible equilibrium reaction. The L-ribose isomerase can be obtained from microorganisms of the genus Acinetobacter.
Claims5
An isolated L-ribose isomerase which isomerizes L-ribose into L-ribulose and vice versa, and which is obtainable from a microorganism selected from the group consisting of Acinetobacter calcoaceticus LR7C (FERM BP-5335) and mutants thereof.
An isolated L-ribose isomerase which isomerizes L-ribose into L-ribulose and vice versa, having the following physicochemical properties:
- (a) Molecular weight About 25,000-35,000 daltons on sodium sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and about 110,000-130,000 daltons on gel filtration method;
- (b) Optimum temperature About 30.degree. C. when incubated at pH 9 for about 10 min;
- (c) Optimum pH A pH of 8-9 when incubated at 30.degree. C. for 10 min;
- (d) Thermal stability Stable up to a temperature of about 30.degree. C. when incubated at pH 9 for 10 min;
- and
- (e) pH stability Stable at a pH of 7-9 when incubated at 4.degree. C. for 24 hours.
An isolated L-ribose isomerase which isomerizes L-ribose into L-ribulose and vice versa, which contains the amino acid sequence of SEQ ID NO:1;
- SEQ ID NO:1:
- Thr Arg Thr Ser Ile.
Dependencies are inferred from the claim text.