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US 5,846,801 AGranted
Thermostable lipase isolated from Pseudomonas solanacearum
AssigneeNovo Nordisk A/S
InventorsTakashi Kotsuka, Keijitsu Tanaka, Kazunori Sakimoto
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Abstract
A thermostable lipase has been isolated from Pseudomonas solanacearum SD709 (FERM BP-5358) which has a mass of 32 kD by SDS-PAGE, enzymatic activity in a pH range of about 4-12, a pH optimum of 6.5-9.5 and a temperature optimum of 80.degree.-90.degree. C.
Claims5
A purified lipase isolated from a strain of Pseudomonas, having:
- (a) a temperature optimum of from 80.degree. to 90.degree. C., determined with triolein emulsion as a substrate in the presence of 5 mM EDTA;
- (b) enzymatic activity in a pH range of about 4-12;
- and
- (c) a pH optimum at 6.5-9.5, determined using triolein emulsion as a substrate.
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Dependencies are inferred from the claim text.