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US 5,846,799 AGranted
Human myeloperoxidase and its therapeutic application
AssigneeLa Region Wallone
InventorsCarol Deby, Joel Pincemail, Alex Bollen
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Abstract
A method of preparing biologically active human myeloperoxidase including the steps of preparing a vector for the expression of human myeloperoxidase. The vector includes plasmid pNIV2703. The plasmid includes a HindIII-SnaBI, HindIII-EcoRV or HindIII-HpaI cassette, the cassette including a complete DNA sequence for hMPO as shown in FIG. 1. CHO cells are transformed with the vector. The cells are cultured. The biologically active human myeloperoxidase is recovered.
Claims6
A method of preparing biologically active human myeloperoxidase, said method comprising the steps of:
- preparing a vector for the expression of human myeloperoxidase, said vector comprising plasmid pNIV2703, said plasmid including a HindIII-SnaBI, HindIII-EcoRV or HindIII-HpaI cassette, said cassette including a complete DNA sequence for hMPO as shown in FIG. 1;
- transforming CHO cells with said vector;
- culturing said cells;
- and
- recovering said biologically active human myeloperoxidase.
2 more elements
Dependencies are inferred from the claim text.