RNA transformation vector
AssigneeMycogen Plant Science, Inc.
InventorsPaul G. Ahlquist, Roy C. French
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Abstract
A +strand RNA viral transformation of host organisms with foreign RNA, and expression of said foreign RNA. The foreign RNA is inserted into an infective RNA viral segment containing replication elements, and allowed to infect the host organism. The invention is exemplified utiliing brome mosaic RNA modified to contain a gene coding for chloramphenicol acetyl transferase (CAT) in the transformation of barley protoplasts.
Claims30
An RNA molecule comprising a cis-acting replication element derived from a (+) strand RNA plant virus capable of replication in a plant cell and further comprising an exogenous RNA segment capable of expressing its function in a host cell, said exogenous RNA segment being located in a region of said RNA molecule able to tolerate said segment without disrupting RNA replication of said RNA molecule in the presence of trans-acting replication elements in said host cell.
A DNA transcription vector comprising cDNA having one strand complementary to an RNA molecule comprising a cis-acting replication element derived from a (+) strand RNA plant virus capable of replication in a plant cell and further comprising an exogenous RNA segment capable of expressing its function in a host cell, said exogenous RNA segment being located in a region of said RNA molecule able to tolerate said segment without disrupting RNA replication of said RNA molecule in the presence of trans-acting replication elements in said host cell.
A method of modifying a host cell, genotypically or phenotypically, which method comprises introducing into the cell an RNA molecule comprising a cis-acting replication element derived from a (+) strand RNA plant virus capable of replication in a plant cell and further comprising an exogenous RNA segment capable of expressing its function in a host cell, said exogenous RNA segment being located in a region of said RNA molecule able to tolerate said segment without disrupting RNA replication of said RNA molecule in the presence of transacting replication elements in said host cell, whereby the exogenous RNA segment confers a detectable trait in the host cell, thereby modifying said host cell.
Dependencies are inferred from the claim text.