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AssigneeAjinomoto Co., Inc.
InventorsEiichiro Kimura, Yoko Asakura, Akinori Uehara, Sumio Inoue, Yoshio Kawahara, Yasuhiko Yoshihara, Tsuyoshi Nakamatsu
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A mutant strain having an ability to produce L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin is obtained by giving temperature sensitivity with respect to a biotin action-suppressing agent to a coryneform L-glutamic acid-producing bacterium. This strain is cultivated in a liquid medium to produce and accumulate L-glutamic acid in the medium. A mutant strain having an ability to produce L-lysine and L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin is obtained by giving temperature sensitivity with respect to a biotin action-suppressing agent and giving L-lysine productivity to a coryneform L-glutamic acid-producing bacterium. This strain is cultivated in a liquid medium to simultaneously produce and accumulate L-lysine and L-glutamic acid in the medium.
A method of producing L-glutamic acid by fermentation without biotin-action suppressive agents, comprising the steps of:
A method of producing L-lysine and L-glutamic acid by fermentation without biotin-action suppressing agents, comprising the steps of:
A mutant strain of coryneform bacteria, having a temperature-sensitive mutation with respect to a biotin action-suppressing agent produced by applying a mutation treatment to a coryneform L-glutamic acid-producing bacterium conducting a replication method in a growth medium containing a biotin-action-suppressing agent and selecting a strain which is temperature sensitive at about 37.degree. C. in said growth medium but not temperature sensitive in media without said biotin-action-suppressing agent, and having the ability to produce L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin.
A method of breeding mutant coryneform strains having the ability to produce L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin, comprising:
A method of breeding mutant coryneform strains having the ability to produce both L-lysine and L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin, comprising:
Dependencies are inferred from the claim text.