Process for preparing nontoxic lipopolysaccharides from acidiphilium species
AssigneeCouncil of Scientific and Industrial Research
InventorsRanjan Bhadra, Abhijit Nayak, Pataki Charan Bandyopadhyay, Sumanta Basu
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Abstract
A process for preparing a nontoxic lipopolysaccharide, which comprises (i) cultivating in a culture medium bacteria of the genus Acidophilium having accession No. MTCC 1979, (ii) separating the culture bacteria, and repeatedly washing the separated bacteria, (iii) extracting the washed bacteria with a lipophilic solvent, (iv) cooling the extract and repeatedly centrifuging the cooled material, (v) dialyzing the supernatant resulting from said centrifuging, (vi) lyophilizing the dialyzed material, (vii) precipitating the lipopolysaccharide by treating the lyophilized material with a polar solvent, and (viii) drying the precipitated polyliposaccharide.
Claims12
A process for preparing a nontoxic lipopolysaccharide, the process comprising:
- (i) cultivating in a culture medium bacteria of the genus Acidiphilium having accession No. ATCC 55963,
- (ii) separating the cultured bacteria, and repeatedly washing the separated bacteria,
- (iii) extracting the washed bacteria with a lipophilic solvent at an elevated temperature,
- (iv) cooling the extract and repeatedly centrifuging the cooled material,
- (v) dialyzing the supernatant resulting from said centrifuging,
- (vi) lyophilizing the dialyzed material,
- (vii) precipitating the lipopolysaccharide by treating the lyophilized material with a polar solvent, and
- (viii) drying the precipitated lipopolysaccharide.
Dependencies are inferred from the claim text.