Oligonucleotides specific for the MOMP gene sequence and methods for the detection of Chlamydia trachomatis
AssigneeAbbott Laboratories
InventorsJohn D. Burczak, John J. Carrino, Paul A. Klonowski, Matthew T. Manlove, Ronald L. Marshall, Edward K. Pabich, John A. Salituro
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Abstract
The present invention relates to oligonucleotide probes useful in detecting, e.g. by hybridization or the ligase chain reaction, Chlamydia trachomatis DNA in the presence of other related DNA. The present invention is also directed to methods of detecting Chlamydia trachomatis organisms in a sample using the ligase chain reaction.
Claims18
A composition for detecting target DNA from Chlamydia trachomatis, said composition comprising a set of four oligonucleotide probes, said set selected from the group consisting of probe set 1 (SEQ ID NOS. 2-5), probe set 2 (SEQ ID NOS. 7-10), and probe set 3 (SEQ ID NOS. 12-15) as defined herein, and combinations thereof, wherein said composition is capable of detecting at least Chlamydia trachomatis serovars D, F, L1 and L2.
A kit useful in detecting target DNA from Chlamydia trachomatis, said kit comprising one or more suitable containers containing:
- a) at least one set of four oligonucleotide probes wherein said probe set is selected from the group consisting of probe set 1 (SEQ ID NOS. 2-5), probe set 2 (SEQ ID NOS. 7-10), and probe set 3 (SEQ ID NOS. 12-15) wherein said probe set is capable of detecting at least Chlamydia trachomatis serovars D, F, L1 and L2;
- b) a polymerase reagent;
- c) one or more but less than all four deoxynucleotide triphosphates;
- and
- d) a ligase reagent.
A composition for detecting target DNA from at least Chlamydia trachomatis serovars D, F, L1 and L2, said composition comprising a pair of oligonucleotide probes, said pair being selected from the group consisting of SEQ ID NOS. 2 and 4, SEQ ID NOS. 3 and 5, SEQ ID NOS. 7 and 9, SEQ ID NOS. 8 and 10, SEQ ID NOS. 12 and 14, and SEQ ID NOS. 13 and 15.
Dependencies are inferred from the claim text.