Chlorella virus promoters
AssigneeBioNebraska, Inc.
InventorYuannan Xia
Life of this patent
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Abstract
The present invention provides novel promoter sequences obtained from Chlorella virus. The invention includes gene constructs comprising a promoter sequence of the invention operably linked to a DNA sequence encoding a structural gene. The invention also provides vectors and host cells for expressing a product encoded by the structural gene of a gene construct of the invention and cells transformed with the heterologous gene operably linked to the promoter.
Claims19
A vector comprising the nucleic acid sequence set forth as SEQ ID NO:
- 1.
A vector comprising the nucleic acid sequence set forth as SEQ ID NO:
- 2.
A vector comprising the nucleic acid sequence set forth as SEQ ID NO:
- 3.
A vector comprising the nucleic acid sequence set forth as SEQ ID NO:
- 4.
A vector comprising the nucleic acid sequence set forth as SEQ ID NO:
- 5.
A vector comprising the nucleic acid sequence set forth as SEQ ID NO:
- 6.
A vector comprising the nucleic acid sequence set forth as SEQ ID NO:
- 7.
A gene construct comprising a promoter operably linked to a heterologous structural gene, said promotor comprising the nucleic acid sequence set forth as SEQ ID NO:
- 1,2,3,4,5,6,or7.
A host cell containing a gene construct comprising a promotor operably linked to a heterologous structural gene, said promotor comprising the nucleic acid sequence set forth as SEQ ID NO:
- 1, 2, 3, 4, 5, 6, or 7.
A process for producing a protein, said process comprising:
- transforming a host cell with a gene construct comprising a promotor operably linked to a structure gene, said promotor comprising a nucleic acid sequence set forth as SEQ ID NO:
- 1,2,3,4,5,6, or 7;and
- culturing the host cell under conditions whereby the protein is expressed.
A process for producing a protein said process comprising:
- incubating a host cell under conditions whereby the protein is expressed, which host cell comprises a gene construct comprising a promotor operably liked to a heterologous nucleic acid sequence encoding the protein, said promoter comprising a nucleic acid sequence as set forth as SEQ ID NO:
- 1, 2, 3, 4, 5, 6, or 7.
Dependencies are inferred from the claim text.