Method of detecting live microorganisms
AssigneeRusteck Limited
InventorsStanislav Fedorovich Rastopov, Vladimir Gennadievich Ageev
Life of this patent
Estimating the term…
Abstract
The invention relates to the field of optical biosensors and facilitates detection of mobile microorganisms in a liquid with a high degree of sensitivity even in the presence of a large number of brownian particles. The method in essence involves measuring, within a frequency range restricted at the lower end, the mean amplitude of the fluctuation in intensity of laser radiation scattered by the particles in the liquid, this being proportional to both the concentration and mobility of the particles. The turbidity of the liquid, which is proportional only to the particle concentration, is also measured and the presence of mobile microorganisms is assessed by comparing the two signals.
Claims3
A method for detecting live microorganisms, comprising passing laser radiation through the sample being analyzed and measuring the parameter characterizing the amplitude-frequency spectrum of fluctuation of the scattered laser radiation in the sample, simultaneously measuring the parameter characterizing the turbidity of the sample, while measuring the parameter characterizing the amplitude-frequency spectrum of fluctuation of scattered laser radiation with a restriction on the lower limit of the frequency range, and detecting the presence of live microorganisms by comparing the measured parameters.
Dependencies are inferred from the claim text.