Quantitative measurement of tissue protein identified by immunohistochemistry and standardized protein determination
AssigneeThe Regents of the University of California
InventorsDennis J. Slamon, Michael F. Press
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Abstract
Cellular components are quantitated using stained cell samples, computerized image analysis, and cellular standards, where the computerized image analysis value can be translated into the amount of the component per cell. The methodology is demonstrated with breast cancer cells and quantitation of the HER-2/neu gene. The quantitation is shown to have prognostic capability as to the future course of the disease.
Claims12
A method for quantitating a surface membrane or cytosol protein, said method comprising:
- (1) staining immobilized fixed cells with a labeled binding composition comprising a probe binding specifically to said surface membrane or cytosol protein, wherein said label can be quantitated by computerized image analysis;
- (2) determining the signal value from a known number of said fixed cells by means of computerized image analysis;
- and
- determining the amount of said surface membrane or cytosol protein per cell by comparing said signal value obtained from said fixed cells with control values between control cellular compositions having said surface membrane or cytosol protein at different cellular amounts for which signal values are obtained in accordance with steps (1) and (2) and for which the amount of said surface membrane or cytosol protein for a known number of cells is obtained by an alternative method of analysis other than computerized image analysis.
A method, for quantitating a molecular cellular component, said method comprising:
- (1) staining immobilized fixed cells with a labeled binding composition comprising a probe binding specifically to said molecular cellular component, wherein said label can be quantitated by computerized image analysis:
- (2) determining the signal value from a known number of said fixed cells by means of computerized image analysis:
- and
- determining the amount of said molecular cellular component per cell by comparing said signal value obtained from said fixed cells with control values between control cellular compositions having said molecular cellular component at different cellular amounts for which signal values are obtained in accordance with steps (1) and (2) and for which the amount of said molecular cellular component for a known number of cells is obtained by an alternative method of analysis other than computerized image analysis, wherein said alternative method of analysis comprises comparing the value obtained from analysis of lysates of said control cellular compositions as compared to the value obtained with pure preparations of said molecular cellular component using the same method of analysis.
A method for quantitating HER-2/neu protein from breast cancer tissue as a prognostic of relapse, said method comprising:
- (1) staining fixed cells from said breast cancer tissue with an enzyme labeled binding composition comprising a probe binding specifically to said HER-2/neu protein, wherein said label can be quantitated by computerized image analysis;
- (2) determining the signal value from a known number of said fixed cells by means of computerized image analysis;
- and
- determining the amount of said HER-2/neu protein per cell by comparing said signal value obtained from said fixed cells with control values between control cellular compositions having said HER-2/neu protein at different cellular amounts for which signal values are obtained in accordance with steps (1) and (2) and for which the amount of said HER-2/neu protein for a known number of cells is obtained by comparing the value obtained from analysis of lysates of said control cellular compositions as compared to the value obtained with pure preparations of said HER-2/neu protein using the same alternative method of analysis other than computerized image analysis.
A kit comprising at least three immortalized cell lines comprising differing amounts of a surface membrane protein or cytosol protein, wherein said immortalized cell lines comprise at least one naturally occurring tumor cell composition;
- and
- an antibody composition that specifically binds to said surface membrane protein or cytosol protein.
Dependencies are inferred from the claim text.