Antibody to ocular and vaginal surface epithelium
AssigneeThe Schepens Eye Research Institute, Inc.
InventorIlene K. Gipson
Life of this patent
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Abstract
A monoclonal antibody has been produced that binds to apical cells of human ocular surface and vaginal epithelia. The antigen recognized by this monoclonal antibody is a mucin-like cell surface glycoprotein. The antibody is of diagnostic use in the evaluation of the ocular surface in dry eye patients, and the antigen has therapeutic potential for use in treatment of dry eye disease. In a comparison of dry eye and normal patients the pattern of antibody binding to dry eye ocular surface epithelia has been shown to differ from the binding pattern seen in normal epithelia.
Claims11
A monoclonal antibody or monoclonal antibody fragment selected from the group consisting of a monoclonal antibody produced by the hybridoma cell line deposited with the American Type Culture Collection (ATCC) as ATCC No. HB 11488, and Fab, F(ab').sub.2 and Fc fragments of the monoclonal antibody produced by hybridoma cell line ATCC No. HB 11488.
A hybridoma cell line deposited with the American Type Culture Collection as ATCC No. HB 11488.
A method of identifying a cell surface glycoprotein antigen from human epithelial tissue that is bound specifically by a monoclonal antibody produced by hybridoma cell line ATCC No. HB 11488, said method comprising
- providing a sample of human epithelial tissue;
- reacting said sample with a detectable amount of a monoclonal antibody produced by hybridoma cell line ATCC No. HB 11488;
- detecting any binding of said monoclonal antibody to an antigen in said sample that is specifically bound by said monoclonal antibody;
- and
- identifying an antigen in said sample that is bound specifically by said monoclonal antibody.
Dependencies are inferred from the claim text.