Conjugates of sulforhodamine fluorophores with enhanced fluorescence
AssigneeMolecular Probes, Inc.
InventorHee Chol Kang
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Abstract
The invention describes useful conjugates of sulforhodamine, wherein the conjugated substance and the fluorophore are separated by an alkanoic acid spacer that is attached to the fluorophore via a sulfonamide bond. The increased length of the covalent linkage due to the spacer results in dye-conjugates having a number of surprisingly advantageous properties relative to previous sulforhodamine-labeled conjugates, including increased fluorescence.
Claims34
A dye-conjugate comprising a conjugated substance that is a peptide, protein, polysaccharide, nucleotide, oligonucleotide or nucleic acid polymer to which is attached one or more dye molecules, which may be the same or different, having the formula ##STR6## or the formula ##STR7## wherein n=1-10;
- R.sup.1 is CH.sub.3 or CH.sub.2 CH.sub.3 ;
- and
- R.sup.2 is the conjugated substance.
A method of detecting a complementary member of a specific binding pair in a sample, comprising:
- a) adding to said sample a dye-conjugate of a first member of a specific binding pair for which there is a complementary member, to which is attached one or more dye molecules, which may be the same or different, having the formula ##STR8## or the formula ##STR9## wherein n=1-10;
- R.sup.1 is CH.sub.3 or CH.sub.2 CH.sub.3 ;
- and
- R.sup.2 is the first member of the specific binding pair;
- b) allowing sufficient time for the dye-conjugate to form a complex with the complementary member;
- and
- c) detecting the complex to locate the complementary member.
A complex of a dye-conjugate of a first member of a specific binding pair and a complementary member of said complementary member, where the first member of the specific binding pair is attached to one or more dyes, which may be the same or different, having the formula ##STR10## or the formula ##STR11## wherein n=1-10;
- R.sup.1 is CH.sub.3 or CH.sub.2 CH.sub.3 ;
- and
- R.sup.2 is the first member of the specific binding pair.
A dye-conjugate, prepared by the reaction of a substance that is a peptide, protein, polysaccharide, nucleotide, oligonucleotide or nucleic acid polymer with a reactive dye having the formula ##STR12## or the formula ##STR13## or a mixture thereof, wherein n=1-10;
- and
- R.sup.1 is CH.sub.3 or CH.sub.2 CH.sub.3.
Dependencies are inferred from the claim text.