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US 5,846,736 AGranted
Seed storage protein with nutritionally balanced amino acid composition
OwnerAsis Datta, Anjana Raina +1inventors
InventorsAsis Datta, Anjana Raina, Subhra Biswas
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Abstract
The present invention relates, in general to protein that is a seed storage protein having high nutritional value. In particular, the invention relates to the protein AmA1 and to a DNA sequence encoding same. The invention further relates to a recombinant molecule comprising the AmA1 encoding sequence and to a host cell transformed therewith. In addition, the invention relates to a method for producing transgenic plants with high nutritionally rich amino acids.
Claims5
A process for the preparation of DNA encoding the seed specific AmA1 protein from Amaranthus which comprises:
- i) isolating messenger RNA from Amaranthus by conventional methods;
- ii) preparing from mRNA double stranded cDNA with reverse transcriptase using oligo (dT) primers;
- iii) combining the double stranded cDNA with an expression vector;
- iv) isolating from the resulting recombinant vectors those encoding said seed specific protein by conventional methods;
- and
- v) sequencing the DNA encoding said seed specific protein.
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Dependencies are inferred from the claim text.