Structural phosphoprotein (PP150) of human cytomegalovirus, and the preparation and use thereof
AssigneeBehring Diagnositcs GmbH
InventorsGerhard Jahn, Birgit-Christine Scholl, Michael Broker, Michael Mach, Bernhard Fleckenstein, Bernd Traupe
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Abstract
The phosphorylated structural protein of molecular weight about 150 kd (pp 150) of human cytomegalovirus (HCMV) is highly immunogenic and is reliably recognised by human antisera. This protein can, after assignment and sequencing of the gene, be prepared, in whole or in immunogenic sections, by gene manipulation. Proteins of this type are suitable as reagents, for example in an ELISA, and as constituents of vaccines.
Claims8
A recombinant polypeptide, obtained by expression of a DNA molecule encoding the HCMV structural phosphoprotein with a molecular weight of 150 kd (pp 150), which is located in the region of the fragments HindIII J/N of the genome of HCMV, strain Ad 169, in a nonhuman cell, wherein the polypeptide will bind to an antibody directed against HCMV.
A recombinant polypeptide, obtained by expression of a DNA molecule encoding the HCMV structural phosphoprotein with a molecular weight of 150 kd (pp 150), which is located on a 1.5 kb EcoRI-PstI fragment inside the EcoRI Y fragment from the genome of HCMV, strain Ad 169, in a nonhuman cell, wherein the polypeptide will bind to an antibody directed against HCMV.
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