Peptides of caseinomacropeptide, antibodies against the said peptides, and uses
AssigneeSanofi Diagnostics Pasteur
InventorsJean-Claude Collin, Jean-Louis Perrod
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Abstract
The present invention presents an antigenic peptide whose amino acid sequence comprises a fragment of the caseinomacropeptide (CMP) sequence, which peptide is characterized in that it carries at least one epitope of CMP. These peptides exhibit no or little cross-reactivity with K-casein. When these peptides are used as immunogens, they make it possible to obtain specific anti-CMP antibodies. Also disclosed is a process for detecting the presence of cow's milk CMP in milk and milk products, using the above antibodies. Further presented are diagnostic reagents for the assay of CMP, which reagents are characterized in that they comprise at least one peptide, antigenic composition, or anti-CMP antibody of the present invention.
Claims18
An antigenic peptide chosen from the group consisting of peptides having any of the following sequences:
- Met-Ala-Ile-Pro-Pro-Lys (SEQ. ID NO.:
- 7);
- Met-Ala-Ile-Pro-Pro-Lys-Lys (SEQ. ID NO.:
- 8);
- Met-Ala-Ile-Pro-Pro-Lys-Lys-Asn (SEQ. ID NO.:
- 9);
- Met-Ala-Ile-Pro-Pro-Lys-Lys-Asn-Gln (SEQ. ID NO.:
- 10);
- Met-Ala-Ile-Pro-Pro-Lys-Lys-Asn-Gln-Asp (SEQ. ID NO.:
- 11);
- Met-Ala-Ile-Pro-Pro-Lys-Lys-Asn-Gln-Asp-Lys (SEQ. ID No. 6);
- and
- comprising a cysteine linked at its C-terminal end through a peptide linkage.
An antigenic peptide consisting of a fragment of the caseinomacropeptide sequence having the following sequence:
- Ser-Pro-Pro-Glu-Ile-Asn-Thr-Val-Gln-Val (SEQ ID NO:2)
- and comprising a cysteine linked at its C-terminal end through a peptide linkage.
Dependencies are inferred from the claim text.