Oligonucleotide-polyamide conjugates
AssigneeHoward Florey Institute of Experimental Physiology and Medicine
InventorsJim Haralambidis, Geoffrey William Tregear
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Abstract
Methods are provided for the hybridization of a target polynucleotide with a conjugate. The conjugate comprises an oligonucleotide covalently bound to a linker that is covalently bound to an amino acid or a polyamide. The oligonucleotide portion of the conjugate comprises an anti-sense sequence which is complementary to and hybridizes with the target polynucleotide. The method is useful for detecting the presence of viral or bacterial target polynucleotides.
Claims11
A method for hybridizing to a target polynucleotide which comprises contacting an oligonucleotide-polyamide conjugate of the formula X--L--Y with said polynucleotide, wherein:
- X is a single amino acid or a polyamide comprising two or more amino acid residues,
- Y is an oligonucleotide, and
- L is a linker covalently bound to the amino-terminus of said single amino acid or polyamide X and to the 3' phosphate group of said oligonucleotide Y, and wherein said oligonucleotide portion of the conjugate comprises an anti-sense sequence which is complementary to and hybridizes with the target polynucleotide.
Dependencies are inferred from the claim text.