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US 5,846,714 AGranted
Method of identifying a chemical that alters dissociation of an NF-KB/IKB complex
AssigneesChiron Corporation, Unversity of North Carolina
InventorsJohn Stephen Haskill, Albert S. Baldwin, Jr., Peter Ralph
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Abstract
Compositions and methods of using the same are described that have applications for the identification of prophylactics or therapeutics for the treatment of diseases resulting from altered gene expression, including genes that encode cytokines or related molecules.
Claims6
A method of identifying a chemical that increases dissociation of an NF-.kappa.B/I.kappa.B complex, comprising the steps of:
- a) expressing a nucleic acid encoding an I.kappa.B protein having the sequence set forth as SEQ ID NO:
- 18;
- b) purifying said I.kappa.B protein;
- c) preparing a complex comprising said I.kappa.B protein and an NF-.kappa.B protein and contacting the complex with said chemical;
- and
- d) identifying said chemical as a chemical that increases dissociation of said NF-.kappa.B/I.kappa.B complex by its capacity to dissociate the complex of step c).
3 more elements
A method of identifying a chemical that decreases dissociation of an NP-.kappa.B/.kappa.B complex, comprising the steps of:
- a) expressing a nucleic acid encoding an I.kappa.B protein having the sequence set forth as SEQ ID NO:
- 18;
- b) purifying said I.kappa.B protein;
- c) combining in solution an NF-.kappa.B protein, said I.kappa.B protein, and said chemical, said I.kappa.B protein and said NF-.kappa.B protein being present in amounts sufficient to form a complex comprising said I.kappa.B protein and i NF-.kappa.B protein;
- and
- d) identifying said chemical as a chemical that decreases dissociation of said NF-.kappa.B/I.kappa.B complex by its capacity to prevent or retard the dissociation of aid I.kappa.B protein from the complex of step c).
3 more elements
Dependencies are inferred from the claim text.