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US 5,846,713 AGranted
High affinity HKGF nucleic acid ligands and inhibitors
AssigneeNeXstar Pharmaceuticals, Inc.
InventorsNikos Pagratis, Larry Gold
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Abstract
Methods are described for the identification and preparation of high-affinity nucleic acid ligands to hKGF. Included in the invention are specific RNA ligands to hKGF identified by the SELEX method. Also included are RNA ligands that inhibit the interaction of hKGF with its receptor.
Claims6
A method of identifying nucleic acid ligands to Human Keratinocyte Growth Factor (hKGF), comprising:
- a) preparing a candidate mixture of nucleic acids;
- b) contacting the candidate mixture of nucleic acids with hKGF, wherein nucleic acids having an increased affinity to hKGF relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
- c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;
- and
- d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to hKGF, whereby nucleic acid ligands of hKGF may be identified.
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Dependencies are inferred from the claim text.