Nucleotide sequence of two circular SSDNA associated with banana bunchy top virus and method for detection of banana bunchy top virus
AssigneeDevelopment Center for Biotechnology
InventorsRey-Yuh Wu, Li-Ru You, Tai-Seng Soong
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Abstract
Nucleotide sequences of two circular single-stranded DNAs associated with Banana Bunchy Top Virus and the proteins encoded by the open reading frames in the nucleotide sequences are provided. A method for detecting Banana Bunchy Top Virus using the PCR technique based on the nucleotide sequences is also provided.
Claims12
An isolated DNA molecule comprising a nucleotide sequence encoding a polypeptide comprising amino acid sequence SEQ ID NO:
- 16, wherein said sequence is selected from the group consisting of:
- (a) nucleotide sequence SEQ ID NO:
- 3;
- (b) fragments of nucleotide sequence SEQ ID NO:
- 3 that selectively hybridize to Banana Bunchy Top Virus (BBTV) DNA;
- and
- (c) nucleotide sequences which, as a result of the aenetic code, are degenerate to the sequences of (a) or (b).
An isolated DNA molecule comprising a nucleotide sequence encoding a polypeptide comprising amino acid sequence SEQ ID NO:
- 17, wherein said sequence is selected from the group consisting of:
- (a) nucleotide sequence SEQ ID NO:
- 4;
- (b) fragments of nucleotide sequence SEQ ID NO. 4 that selectively hybridize to Banana Bunchy Top Virus (BBTV) DNA;
- and
- (c) nucleotide sequences which, as a result of the aenetic code, are degenerate to the sequences of (a) or (b).
An isolated polypeptide, wherein said polypeptide is encoded by an open reading frame in a nucleotide sequence which is a subsequence of the Banana Bunchy Top Virus (BBTV) genome, said nucleotide sequence being nucleotide sequence SEQ ID NO:
- 3, or a nucleotide sequence which, as a result of the genetic code, is degenerate thereto.
An isolated polyoeptide, wherein said polypeptide is encoded by an open reading frame in a nucleotide sequence which is a subsequence of the Banana Bunchy Top Virus (BBTV) genome, said nucleotide sequence being nucleotide sequence SEQ ID NO:
- 4, or a nucleotide sequence which, as a result of the genetic code, is degenerate thereto.
An isolated DNA molecule comprising a nucleotide sequence encoding a fragment of a component of the Banana Bunchy Top Virus (BBTV) genome, wherein said sequence is selected from the group consisting of:
- (a) nucleotide sequence SEQ ID NO. 1
- (b) fragments of nucleotide sequence SEQ ID NO. 1 that selectively hybridize to Banana Bunchy Top Virus (BBTV) DNA;
- and
- (c) nucleotide sequences which are degenerate to the sequences of (a) or (b).
A method for detecting Banana Bunchy Top Virus (BBTV) in plant tissues comprising the steps of:
- (a) extracting the total DNA of the plant tissues or the DNA of viruses contained in the plant tissues;
- (b) selecting an inversely oriented primer pair from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3 and SEQ ID NO:
- 4;
- (c) performing polymerase chain reaction (PCR) using the extracted DNA as template and the selected primer pair to obtain a PCR product;
- and
- (d) determining the size of the PCR product obtained, wherein a DNA fragment of 1.1 Kb is indicative of the presence of BBTV in the plant tissues.
Dependencies are inferred from the claim text.