Method for suppressing inhibition of enzyme-mediated reactions by ionic detergents using high concentration of non-ionic detergent
AssigneeGen-Probe Incorporated
InventorsDaniel Louis Kacian, Diane Lisa McAllister
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Abstract
Methods and kits for the use of a non-ionic detergent to suppress enzyme inhibition in a reaction solution due to the presence of inhibiting ionic detergent. Prior to reaction, the reaction mixture is given an effective amount of a non-ionic detergent, and agitated. The enzyme is then added, and the enzymatic reaction is the allowed to proceed. Also disclosed are preferred embodiments of the present invention, and kits for nucleic acid amplification of a biological sample in the presence of an ionic detergent.
Claims32
A method for preventing inhibition of an enzymatic activity by an ionic detergent, comprising the steps of:
- a) providing, to a medium containing at least one ionic detergent present in an amount sufficient to inhibit an enzymatic activity if an enzyme comprising said enzymatic activity were present,
- i) at least one non-ionic detergent to a final concentration of from about 6% to about 20%, and;
- b) adding said enzyme comprising said enzymatic activity to the resulting mixture.
Dependencies are inferred from the claim text.