Method of binding collagen recognizing substances to liposomes
AssigneeBaxter International Inc.
InventorRimona Margalit
Life of this patent
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Abstract
Recognizing substances, including epidermal growth factor, gelatin, collagen and hyaluronic acid, have been covalently bound to liposomal surfaces and utilized to attach liposomes onto a cellular or an extracellular matrix (ECM) target site. These "bioadhesive" liposomes offer several advantages including the mutual protection of both the drug and biological environment; an increase in drug bioavailability and retention at the target site; and improved adherence or adhesion to the designated target site.
Claims12
A process for creating a bioadhesive liposome formed by covalently bonding a recognizing substance to a liposome comprising the steps of:
- (a) providing a reaction vessel containing a liposome having phosphatidylethanolamine;
- (b) admixing a recognizing substance of collagen to the reaction vessel to form a reaction mixture with the liposome;
- (c) buffering the reaction mixture;
- (d) admixing a crosslinking reagent to the buffered reaction mixture;
- (e) incubating the buffered reaction mixture and reagent for a period of time sufficient to form the covalent bonding of amine residues of the collagen recognizing substance and liposome to create a bioadhesive liposome;
- and,
- (f) separating and removing excess unreacted and noncovalently bound materials from the bioadhesive liposome.
A bioadhesive liposome designed for the sustained release of a therapeutic substance comprising a liposome component, the therapeutic substance encapsulated by the liposome component, and a collagen recognizing substance component bonded to the liposomal surface.
A modified liposome comprising a liposome having phosphatidylethanolamine as a provider of a primary amine residue covalently linked by a crosslinking reagent to a target recognizing substance which is collagen.
Dependencies are inferred from the claim text.