Preparation and use of viral vectors for mixed envelope protein immunogenic composition against human immunodeficiency viruses
AssigneeSt. Jude Children's Research Hospital
InventorsJulia Hurwitz, Karen Slobod
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Abstract
Polyenv immunogenic composition are provided that comprise mixtures of at least 4 different recombinant viruses that each express a different HIV env variant or a portion thereof containing both constant and variable regions, as well as methods of making and using such polyenv immunogenic composition and viruses. The immunogenic composition of the invention are optimally combined with a recombinant HIV env booster, or a recombinant HIV env gene DNA priming or boosting vaccine.
Claims40
An immunogenic composition that can elicit an immune response to more than one but not necessarily all of the env variants contained in the composition, comprising at least 4 different recombinant viruses, each comprising an env variant (EV) nucleotide encoding a different envelope protein variant of a human immunodeficiency virus (HIV) envelope protein, wherein
- a) the EV nucleotide encodes both variable and constant regions of the envelope prote in variant;
- and
- b) the immunogenic composition can elicit at least one of a cellular and a humoral immune response in a mammal against an HIV strain.
A method for making an immunogenic composition that can elicit an immune response to more than one but not necessarily all of the env variants contained in the composition, comprising combining in admixture at least 4 different recombinant viruses to obtain an immunogenic composition, wherein
- i) each of the recombinant viruses comprises an env variant (EV) nucleotide encoding a different envelope protein variant of an HIV envelope protein;
- ii) the EV nucleotide encodes both variable and constant reglions of the envelope protein variant;
- and
- iii) the immunogenic composition can elicit at least one of a cellular and a hiumoral immune response in a mammnal against an HIV strain.
A method for eliciting a humoral or cellular immune response, or both, to a human immunodeficiency virus (HIV) in a maimmal, comprising administering to the mammal an effective amount of an immnunogenic composition that can elicit an immune response to more than one but not necessarily all of the env variants contained in the composition comprising at least 4 different recombinant viruses, wherein
- a) each of the recombinant viruses comprises an env variant (EV) nucleotide encoding a different envelope protein variant of an HIV envelope protein;
- b) the EV nucleotide encodes both variable and constant regions of the envelope protein variant;
- and
- c) the amount of the immunogenic composition is effective to elicit at least one of a cellular and a humoral immune response in the mammal against an HIV strain infecting the mammnal.
An immunogenic composition that can elicit an immune response to more than one but not necessarily all of the env variants contained in the composition, comprising at least 4 different DNA vectors, each DNA vector comprising an env variant (EV) nucleotide encoding a different envelope protein variant of a human immunodeficiency virus (HIV) envelope protein, wherein
- a) the EV nucleotide encodes both variable and constant regions of the envelope protein variant;
- and
- b) the inmmunogenic composition can elicit at least one of a cellular and a humoral immune response in a mammal against an HIV strain.
A method for making an immunogenic composition that can elicit an immune response to more than one but not necessarily all of the env variants contained in the composition, comprising combining in admixture at least 4 different DNA vectors to obtain an immunogenic composition, wherein
- i) each of the DNA vectors comprises an env variant (EV) nucleotide encoding a different envelope protein variant of an HIV envelope protein;
- ii) the EV nucleotide encodes both variable and constant regions of the envelope protein variant;
- and
- iii) the immunogenic composition can elicit at least one of a cellular and a humoral immune response in a mammal against an HIV strain.
A method for eliciting a humoral or cellular immune response, or both, to a human immunodeficiency virus (HIV) in a mammal, comprising administering to the mammal an effective amount of an immunogenic composition that can elicit an immune response to more than one but not necessarily all of the env variants contained in the composition comprising at least 4 different DNA vectors, wherein
- i) each of the DNA vectors comprises an env variant (EV) nucleotide encoding a different envelope protein variant of an HIV envelope protein;
- ii) the EV nucleotide encodes both variable and constant regions of the envelope protein variant;
- and
- iii) the immunogenic composition can elicit at least one of a cellular and a humoral immune response in a maimmal against an HIV strain.
Dependencies are inferred from the claim text.