Immunogenic chimeras comprising nucleic acid sequences encoding endoplasmic reticulum signal sequence peptides and at least one other peptide, and their uses in vaccines and disease treatments
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsNicholas P. Restifo, Steven A. Rosenberg, Jack R. Bennink, Igor Bacik, Jonathan W. Yewdell
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Abstract
Immunogenic chimeric proteins comprising an endoplasmic reticulum signal sequence and at least one other peptide are disclosed. The invention relates to the design of vaccinia virus constructs capable of directing host organism synthesis of immunogenic chimeric proteins which can be used as immunogens, as vaccines, or in methods of treatment for cancer, infectious diseases, or autoimmune diseases.
Claims24
A DNA construct which directs host organism synthesis of an immunogenic chimeric protein, said DNA construct having a nucleic acid sequence comprising:
- (a) a first sequence encoding an endoplasmic reticulum signal sequence peptide;
- and
- (b) a second sequence encoding at least one other peptide selected from the group consisting of tumor peptides, bacterial peptides, parasitic peptides and autoimmune disease peptides, where said other peptide forms a complex with class I MHC molecules.
A DNA construct which directs host organism synthesis of an immunogenic chimeric protein, said DNA construct having a nucleic acid sequence comprising:
- (a) a first sequence encoding an endoplasmic reticulum signal sequence peptide;
- and
- (b) a second sequence encoding at least one viral peptide selected from the group consisting of an adenovirus EIA peptide, an SV40 T antigen peptide, an Epstein Barr virus peptide, and a vesicular stomatitis virus peptide.
Dependencies are inferred from the claim text.