Modified avidin and streptavidin and methods of use thereof
AssigneeUniversity of Rochester
InventorScott F. Rosebrough
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Abstract
The present invention provides modified avidin and streptavidin compounds that have suitable blood clearance kinetics for use in a two-step approach to deliver a molecule to a target site. In particular, to hasten SA's blood clearance carbohydrate moieties are covalently bonded to SA. To prolong Avid's blood clearance, Avid is deglycosylated and/or neutralized by alkylation of its lysine amino acids. In a two-step approach, biotinylated compounds are used to deliver radionuclides, cytotoxic drugs, MRI agents, fluorochromes and other agents suitable for imaging and therapy to target-bound modified streptavidin or avidin conjugated antibodies or other targeting agents.
Claims15
A conjugate of a targeting agent and a covalently modified streptavidin, wherein said covalently modified streptavidin contains at least one carbohydrate moiety, said carbohydrate moiety being galactose, mannose, fructose or lactose which is covalently attached to the streptavidin.
A conjugate of a trigalactose modified streptavidin and a monoclonal antibody, said trigalactose-modified streptavidin (SA) having the formula ##STR5## wherein n is 1 to 20 and gal is galactosyl.
Dependencies are inferred from the claim text.