Ultrasonic direct fixing of reagent layer and method for preparing peel type test piece

A method for preparing a peel type test piece for dry analysis for determining a specific component in a liquid specimen comprising a supporting base plate having thereon a reagent layer comprising a porous matrix impregnated with a reagent and further having thereon a releasable film layer having a function of filtering out corpuscles, the releasable film layer being to be stripped after application of a specimen to observe coloration of the reagent layer, which consists essentially of the steps of:

• superposing the reagent layer and the film layer on the supporting base plate in this order in mutual contact and applying ultrasonic vibrations from the supporting base plate side;
• applying pressure to make the surface of a molten layer encroach and bond to the surface of an adjacent non molten layer or to integrate the surface material of a molten layer with the surface of an adjacent layer;
• and
• removing the ultrasonic vibrations and pressure.

A method for fixing a reagent layer directly onto a supporting base in the preparation of a dry analysis kit for determining a specific component in a liquid specimen, wherein at least one of the reagent layer and the base plate is a thermoplastic resin, which consists essentially of steps of:

• (a) placing the reagent layer in contact with a plurality of discrete points on the base plate and externally applying ultrasonic vibrations and pressure to the two layers to generate functional heat thereby to melt the surface of the thermoplastic resin;
• (b) applying pressure to make the molten surface of the thermoplastic resin encroach and bond to the non-thermal plastic material at the plurality of discrete points or to integrate a plurality of discrete points on the surfaces of thermoplastic layer and base plate;
• and
• (c) removing the ultrasonic vibrations and pressure.